Journal: International Journal of Nephrology
Article Title: Effect of the Direct Renin Inhibitor Aliskiren on Urinary Albumin Excretion in Spontaneous Type 2 Diabetic KK- A y Mouse
doi: 10.1155/2013/519130
Figure Lengend Snippet: Expression of MMP-2, MMP-9, TIMP-1, TIMP-2, fibronectin, type IV collagen, MCP-1, and (P)RR in the kidneys of each mouse using real-time PCR (A: untreated KK mice at 8 weeks of age, B: untreated KK mice at 12 weeks of age, C: untreated KK- A y mice at 8 weeks of age, D: untreated KK- A y mice at 12 weeks of age, and E: treated KK- A y mice at 12 weeks of age). The ratio of MMP-2 (a), MMP-9 (b), TIMP-1 (c), TIMP-2 (d), fibronectin (e), type IV collagen (f), MCP-1 (g), and (P)RR (h) mRNAs to GAPDH mRNA is shown in the kidneys of mice from each group. These expressions were increased significantly in group 4 compared with groups 1, 2, and 3. This increase was attenuated in group 5 (* P < 0.001 versus untreated KK- A y mice at 12 weeks of age).
Article Snippet: The primary antibodies (Abs) were as follows: goat polyclonal anti-MMP-2 Ab (R&D Systems, Inc., Minneapolis, MN, USA), goat polyclonal anti-MMP-9 Ab (R&D Systems, Inc.), rabbit polyclonal anti-TIMP-1 Ab (Abbiotec, LLC, San Diego, CA, USA), rabbit polyclonal anti-TIMP-2 Ab (Abbiotec, LLC), and rat monoclonal anti-F4/80 Ab (MAC497GA; Serotec, Oxford, UK).
Techniques: Expressing, Real-time Polymerase Chain Reaction